Vancomycine irwanya Enterococcus na Gene irwanya ibiyobyabwenge

Ibisobanuro bigufi:

Iki gikoresho gikoreshwa mukumenya neza enterococcus irwanya vancomycine (VRE) hamwe na genes zayo zirwanya ibiyobyabwenge VanA na VanB mumyanya yabantu, amaraso, inkari cyangwa koloni nziza.


Ibicuruzwa birambuye

Ibicuruzwa

Izina RY'IGICURUZWA

HWTS-OT090-Vancomycine irwanya Enterococcus hamwe nigikoresho cyo kurwanya ibiyobyabwenge (Fluorescence PCR)

Epidemiologiya

Kurwanya ibiyobyabwenge bizwi kandi nko kurwanya ibiyobyabwenge, bivuga kurwanya bagiteri kurwanya imiti ya antibacterial.Iyo imiti imaze kurwanya, imiti ya chimiotherapie yibiyobyabwenge izagabanuka cyane.Kurwanya Ibiyobyabwenge bigabanijwemo kurwanya imbere.Kurwanya imbere kugenwa na bagiteri chromosomal genes, yagiye ikurikirana ibisekuruza, kandi ntabwo bizahinduka.Kurwanya kurwanya biterwa nuko nyuma yo guhura na antibiotike, bagiteri zihindura inzira zazo za metabolike kugirango ziticwa na antibiotike.

Indwara ya vancomycine irwanya VanA na VanB iboneka mu kurwanya ibiyobyabwenge, muri byo VanA ikerekana urugero rwinshi rwo kurwanya vancomycine na teicoplanine, VanB yerekana urwego rutandukanye rwo kurwanya vancomycine, kandi ikumva teicoplanine.Vancomycine ikunze gukoreshwa mu buvuzi mu kuvura indwara ziterwa na Gram-positif, ariko kubera ko hagaragaye enterococci irwanya vancomycine (VRE), cyane cyane enterococcus faecalis na enterococcus faecium, ikaba irenga 90%, yazanye ibibazo bikomeye mu kuvura amavuriro; .Kugeza ubu, nta muti wihariye wa antibacterial wo kuvura VRE.Ikirenze ibyo, VRE irashobora kandi kwanduza ingirabuzima fatizo zanduza ibiyobyabwenge izindi enterococci cyangwa izindi bagiteri nziza.

Umuyoboro

FAM Vancomycine irwanya enterococci (VRE): Enterococcus faecalis na Enterococcus faecium
VIC / HEX Igenzura ryimbere
CY5 vancomycin irwanya gene VanB
ROX vancomycin irwanya gene VanA

Ibipimo bya tekiniki

Ububiko Amazi: ≤-18 ℃
Ubuzima bwa Shelf Amezi 12
Ubwoko bw'icyitegererezo spumum, amaraso, inkari cyangwa koloni nziza
CV ≤5.0%
Ct ≤36
LoD 103CFU / mL
Umwihariko Nta reaction-reaction hamwe nizindi ndwara zubuhumekero nka klebsiella pneumoniae, acinetobacter baumannii, pseudomonas aeruginosa, streptococcus pneumoniae, neisseria meningitidis, staphylococcus aureus, klebsiella oxytoca, haemophilus cherichia coli, pseudomonas fluorescens, candida albicans, chlamydia pneumoniae, adenovirus yubuhumekero, cyangwa ingero zirimo izindi gen zirwanya ibiyobyabwenge CTX, mecA, SME, Ingero za SHV na TEM.
Ibikoresho bikoreshwa Ikoreshwa rya Biosystems 7500 Sisitemu-Igihe nyacyo PCR

SLAN-96P Sisitemu nyayo-PCR

LightCycler®480 Sisitemu nyayo-PCR

Urujya n'uruza rw'akazi

Gusabwa gukuramo reagent: Macro & Micro-Test Genomic ADN Kit Kit (HWTS-3014-32, HWTS-3014-48, HWTS-3014-96) na Macro & Micro-Test Automatic Nucleic Acide (HWTS-3006C, HWTS-3006B) .


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