Ibicurane bya SARS-CoV-2 A ibicurane B Acide Nucleic Acide

Ibisobanuro bigufi:

Iki gikoresho gikwiranye na vitro yujuje ubuziranenge bwa SARS-CoV-2, ibicurane A na grippe B nucleic acide ya nasopharyngeal swab na oropharyngeal swab icyitegererezo mu bantu bakekwagaho kwandura SARS-CoV-2, ibicurane A na grippe. B.


Ibicuruzwa birambuye

Ibicuruzwa

Izina RY'IGICURUZWA

HWTS-RT060A-SARS-CoV-2 ibicurane A grippe B Nucleic Acide Ikomatanyirijwe hamwe (Fluorescence PCR)

Epidemiologiya

Indwara ya Corona Virusi 2019 (COVID-19) iterwa na SARS-CoV-2 ikaba β Coronavirus yo mu bwoko.COVID-19 ni indwara yandurira mu myanya y'ubuhumekero, kandi muri rusange abantu barashobora kwandura.Kugeza ubu, abarwayi ba SARS-CoV-2 ni bo soko nyamukuru yandura, kandi abarwayi badafite ibimenyetso na bo bashobora kuba intandaro yo kwandura.Ukurikije iperereza ryakozwe muri iki gihe, igihe cyo gukora ni iminsi 1-14, cyane cyane iminsi 3-7.Ibyagaragaye cyane ni umuriro, inkorora yumye n'umunaniro.Abarwayi bake bafite ibimenyetso nko kuzunguruka mu mazuru, izuru ritemba, kubabara mu muhogo, myalgia na diyare.

Ibicurane ni indwara ikabije y'ubuhumekero iterwa na virusi ya grippe.Yanduye cyane kandi ikwirakwira cyane binyuze mu gukorora no kwitsamura.Ubusanzwe itangira mu mpeshyi no mu itumba.Hariho ubwoko butatu bwibicurane, ibicurane A (IFV A), ibicurane B (IFV B) na ibicurane C (IFV C), byombi ni umuryango wa ortomyxovirus.Ibicurane A na B, ni virusi imwe ya virusi ya RNA, ni yo mpamvu nyamukuru itera indwara zabantu.Ibicurane A ni indwara yandurira mu myanya y'ubuhumekero, harimo H1N1, H3N2 n'ubundi bwoko bworoshye, biroroshye guhinduka.icyorezo ku isi, "shift" bivuga ihinduka rya grippe A, bikavamo virusi "subtype".Ibicurane B bigabanyijemo imirongo ibiri: Yamagata na Victoria.Ibicurane B bifite gusa antigenic drift, kandi birinda gukurikiranwa no kurandurwa na sisitemu y’umubiri y’umuntu binyuze muri mutation.Ariko virusi ya grippe B ihinduka gahoro gahoro kuruta ibicurane byabantu A, nayo itera indwara zubuhumekero nicyorezo mubantu.

Umuyoboro

FAM

SARS-CoV-2

ROX

IFV B.

CY5

IFV A.

VIC (HEX)

Imbere yo kugenzura imbere

Ibipimo bya tekiniki

Ububiko

Amazi: ≤-18 ℃ Mu mwijima

Lyophilisation: ≤30 ℃ mu mwijima

Ubuzima bwa Shelf

Amazi: amezi 9

Lyophilisation: amezi 12

Ubwoko bw'icyitegererezo

Nasopharyngeal swabs, Oropharyngeal swabs

Ct

≤38

CV

≤5.0%

LoD

Amakopi 300 / mL

Umwihariko

Ibisubizo by'ibizamini byambukiranya imipaka byerekanaga ko ibikoresho byari bihuye na coronavirus ya muntu SARSr- CoV, MERSr-CoV, HcoV-OC43, HcoV-229E, HcoV-HKU1, HCoV-NL63, virusi ya syncytial A na B, virusi ya parainfluenza 1, 2 na 3. Norovirus, virusi ya mumps, virusi ya varicella zoster, Mycoplasma pneumoniae, Chlamydia pneumoniae, Legionella, pertussis, ibicurane bya Haemophilus, Staphylococcus aureus, Streptococcus pneumoniae, Streptococcus pyogenes, Klebsiella pneogenes Candida albicans, Candida glabrata Nta reaction yambutse hagati ya Pneumocystis yersini na Cryptococcus neoformans.

Ibikoresho bikoreshwa:

Irashobora guhuza ibikoresho nyamukuru bya fluorescent PCR kumasoko.

Ikoreshwa rya Biosystems 7500 Sisitemu-Igihe nyacyo PCR

Ikoreshwa rya Biosystems 7500 Byihuse-Igihe-Sisitemu ya PCR

QuantStudio®5 Sisitemu-Igihe nyacyo PCR

SLAN-96P Sisitemu nyayo-PCR

LightCycler®480 Sisitemu nyayo-PCR

LineGene 9600 Yongeyeho Sisitemu-Igihe Cyuzuye PCR

MA-6000-Igihe-Cyuzuye Cyumubare Wubushyuhe

BioRad CFX96 Sisitemu-Igihe Cyuzuye PCR

BioRad CFX Opus 96 Sisitemu Yigihe-PCR

Urujya n'uruza rw'akazi

Ihitamo 1.

Gusabwa gukuramo reagent: Macro & Micro-Test Virus ADN / RNA Kit (HWTS-3001, HWTS-3004-32, HWTS-3004-48) na Macro & Micro-Test Automatic Nucleic Acide Extractor (HWTS-3006).

Icya 2.

Gusabwa gukuramo reagent: Gukuramo Acide Nucleic Acide cyangwa Isukura Reagent (YDP302) na Tiangen Biotech (Beijing) Co, Ltd.


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