Ibicurane bya SARS-CoV-2 A ibicurane B Acide Nucleic Acide
Izina RY'IGICURUZWA
HWTS-RT060A-SARS-CoV-2 ibicurane A grippe B Nucleic Acide Ikomatanyirijwe hamwe (Fluorescence PCR)
Epidemiologiya
Indwara ya Corona Virusi 2019 (COVID-19) iterwa na SARS-CoV-2 ikaba β Coronavirus yo mu bwoko.COVID-19 ni indwara yandurira mu myanya y'ubuhumekero, kandi muri rusange abantu barashobora kwandura.Kugeza ubu, abarwayi ba SARS-CoV-2 ni bo soko nyamukuru yandura, kandi abarwayi badafite ibimenyetso na bo bashobora kuba intandaro yo kwandura.Ukurikije iperereza ryakozwe muri iki gihe, igihe cyo gukora ni iminsi 1-14, cyane cyane iminsi 3-7.Ibyagaragaye cyane ni umuriro, inkorora yumye n'umunaniro.Abarwayi bake bafite ibimenyetso nko kuzunguruka mu mazuru, izuru ritemba, kubabara mu muhogo, myalgia na diyare.
Ibicurane ni indwara ikabije y'ubuhumekero iterwa na virusi ya grippe.Yanduye cyane kandi ikwirakwira cyane binyuze mu gukorora no kwitsamura.Ubusanzwe itangira mu mpeshyi no mu itumba.Hariho ubwoko butatu bwibicurane, ibicurane A (IFV A), ibicurane B (IFV B) na ibicurane C (IFV C), byombi ni umuryango wa ortomyxovirus.Ibicurane A na B, ni virusi imwe ya virusi ya RNA, ni yo mpamvu nyamukuru itera indwara zabantu.Ibicurane A ni indwara yandurira mu myanya y'ubuhumekero, harimo H1N1, H3N2 n'ubundi bwoko bworoshye, biroroshye guhinduka.icyorezo ku isi, "shift" bivuga ihinduka rya grippe A, bikavamo virusi "subtype".Ibicurane B bigabanyijemo imirongo ibiri: Yamagata na Victoria.Ibicurane B bifite gusa antigenic drift, kandi birinda gukurikiranwa no kurandurwa na sisitemu y’umubiri y’umuntu binyuze muri mutation.Ariko virusi ya grippe B ihinduka gahoro gahoro kuruta ibicurane byabantu A, nayo itera indwara zubuhumekero nicyorezo mubantu.
Umuyoboro
| FAM | SARS-CoV-2 |
| ROX | IFV B. |
| CY5 | IFV A. |
| VIC (HEX) | Imbere yo kugenzura imbere |
Ibipimo bya tekiniki
| Ububiko | Amazi: ≤-18 ℃ Mu mwijima |
| Lyophilisation: ≤30 ℃ mu mwijima | |
| Ubuzima bwa Shelf | Amazi: amezi 9 |
| Lyophilisation: amezi 12 | |
| Ubwoko bw'icyitegererezo | Nasopharyngeal swabs, Oropharyngeal swabs |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | Amakopi 300 / mL |
| Umwihariko | Ibisubizo by'ibizamini byambukiranya imipaka byerekanaga ko ibikoresho byari bihuye na coronavirus ya muntu SARSr- CoV, MERSr-CoV, HcoV-OC43, HcoV-229E, HcoV-HKU1, HCoV-NL63, virusi ya syncytial A na B, virusi ya parainfluenza 1, 2 na 3. Norovirus, virusi ya mumps, virusi ya varicella zoster, Mycoplasma pneumoniae, Chlamydia pneumoniae, Legionella, pertussis, ibicurane bya Haemophilus, Staphylococcus aureus, Streptococcus pneumoniae, Streptococcus pyogenes, Klebsiella pneogenes Candida albicans, Candida glabrata Nta reaction yambutse hagati ya Pneumocystis yersini na Cryptococcus neoformans. |
| Ibikoresho bikoreshwa: | Irashobora guhuza ibikoresho nyamukuru bya fluorescent PCR kumasoko. Ikoreshwa rya Biosystems 7500 Sisitemu-Igihe nyacyo PCR Ikoreshwa rya Biosystems 7500 Byihuse-Igihe-Sisitemu ya PCR QuantStudio®5 Sisitemu-Igihe nyacyo PCR SLAN-96P Sisitemu nyayo-PCR LightCycler®480 Sisitemu nyayo-PCR LineGene 9600 Yongeyeho Sisitemu-Igihe Cyuzuye PCR MA-6000-Igihe-Cyuzuye Cyumubare Wubushyuhe BioRad CFX96 Sisitemu-Igihe Cyuzuye PCR BioRad CFX Opus 96 Sisitemu Yigihe-PCR |
Urujya n'uruza rw'akazi
Ihitamo 1.
Gusabwa gukuramo reagent: Macro & Micro-Test Virus ADN / RNA Kit (HWTS-3001, HWTS-3004-32, HWTS-3004-48) na Macro & Micro-Test Automatic Nucleic Acide Extractor (HWTS-3006).
Icya 2.
Gusabwa gukuramo reagent: Gukuramo Acide Nucleic Acide cyangwa Isukura Reagent (YDP302) na Tiangen Biotech (Beijing) Co, Ltd.
