MTHFR Gene Polymorphic Nucleic Acide

Ibisobanuro bigufi:

Iki gikoresho gikoreshwa mugushakisha imbuga 2 za mutation ya gen ya MTHFR.Igikoresho gikoresha amaraso yumuntu yose nkicyitegererezo kugirango gitange isuzuma ryiza ryimiterere ya mutation.Irashobora gufasha abaganga gutegura gahunda yo kuvura ikwiranye nuburyo butandukanye butandukanye kurwego rwa molekile, kugirango ubuzima bwabarwayi bugerweho cyane.


Ibicuruzwa birambuye

Ibicuruzwa

Izina RY'IGICURUZWA

HWTS-GE004-MTHFR Gene Polymorphic Nucleic Acide Detect Kit (ARMS-PCR)

Epidemiologiya

Acide Folike ni vitamine ikabura amazi ni cofactor ya ngombwa mu nzira zo guhinduranya umubiri.Mu myaka yashize, umubare munini w’ubushakashatsi wemeje ko, ihinduka rya folate metabolizing enzyme gene MTHFR bizatera kubura aside folike mu mubiri, kandi kwangirika kwinshi kubura aside folike ku bantu bakuru bishobora gutera amaraso make ya megaloblastique, imitsi kwangirika kwa endoteliyale, nibindi. Kubura aside folike ku bagore batwite ntibishobora guhaza ibyo bakeneye ubwabo ndetse n’uruyoya, bishobora gutera inenge zifata imitsi, anencephaly, kubyara, no gukuramo inda.Urwego rwa serumu folate rwibasiwe na 5,10-methylenetetrahydrofolate reductase (MTHFR) polymorphism.677C> T na 1298A> C ihindagurika muri gene ya MTHFR itera ihinduka rya alanine kuri valine na aside glutamic, bigatuma ibikorwa bya MTHFR bigabanuka bityo bikagabanya ikoreshwa rya aside folike.

Umuyoboro

FAM MTHFR C677T
ROX MTHFR A1298C
VIC (HEX) Kugenzura imbere

Ibipimo bya tekiniki

Ububiko

≤-18 ℃

Ubuzima bwa Shelf

Amezi 12

Ubwoko bw'icyitegererezo

Amaraso yegeranijwe mashya ya EDTA anticoagulated

CV

≤5.0%

Ct

≤38

LoD

1.0ng / μL

Ibikoresho bikoreshwa:

Ikoreshwa rya Biosystems 7500 Sisitemu-Igihe nyacyo PCR

Ikoreshwa rya Biosystems 7500 Byihuse-Igihe-Sisitemu ya PCR

SLAN ®-96P Sisitemu-Igihe nyacyo PCR

QuantStudio ™ 5 Sisitemu-Igihe nyacyo PCR

LightCycler®480 Sisitemu nyayo-PCR

LineGene 9600 Yongeyeho Sisitemu-Igihe Cyuzuye PCR

MA-6000-Igihe-Cyuzuye Cyumubare Wubushyuhe

BioRad CFX96 Sisitemu-Igihe Cyuzuye PCR

BioRad CFX Opus 96 Sisitemu Yigihe-PCR

Urujya n'uruza rw'akazi

Ihitamo 1

Gusabwa gukuramo reagent: Macro & Micro-Test Genomic ADN Kit Kit (HWTS-3014-32, HWTS-3014-48, HWTS-3014-96) na Macro & Micro-Test Automatic Nucleic Acide (HWTS-3006C, HWTS-3006B) .

Icya 2

Gusabwa gukuramo reagent: Igikoresho cyo gukuramo amaraso ya ADN (YDP348, JCXB20210062) na Tiangen Biotech (Beijing) Co., Ltd.Gukuramo Amaraso ya Genome (A1120) na Promega.


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