KRAS 8 Guhinduka

Ibisobanuro bigufi:

Iki gikoresho kigenewe muri vitro kumenya neza ihinduka ryimiterere 8 muri code ya 12 na 13 za K-ras gene muri ADN yakuwe mubice bya paraffine byashyizwemo na pathologiya.


Ibicuruzwa birambuye

Ibicuruzwa

Izina RY'IGICURUZWA

HWTS-TM014-KRAS 8 Igikoresho cyo Gutahura Ibihinduka (Fluorescence PCR)

HWTS-TM011-Gukonjesha-yumye KRAS 8 Igikoresho cyo Guhindura Ibihinduka (Fluorescence PCR)

Icyemezo

CE

Epidemiologiya

Guhindura ingingo muri gene ya KRAS byagaragaye mubwoko butandukanye bwibibyimba byabantu, hafi 17% ~ 25% ihinduka ryimiterere yibibyimba, 15% ~ 30% ihinduka ryimiterere yabarwayi ba kanseri yibihaha, 20% ~ 50% ihinduka rya kanseri yibara. abarwayi.Kuberako poroteyine ya P21 yashizwemo na gene ya K-ras iherereye hepfo yinzira yerekana inzira ya EGFR, nyuma ya mutation ya K-ras, inzira yerekana ibimenyetso byama nantaryo ikora kandi ntigire ingaruka kumiti yibasiwe na EGFR, bikavamo gukomeza ikwirakwizwa rya selile.Guhinduka kwa gene ya K-ras muri rusange bitanga imbaraga zo kurwanya EGFR tyrosine kinase inhibitor ku barwayi ba kanseri y'ibihaha no kurwanya imiti igabanya ubukana bwa EGFR ku barwayi ba kanseri yibara.Mu mwaka wa 2008, Ikigo cy’igihugu gishinzwe kurwanya kanseri (NCCN) cyasohoye umurongo ngenderwaho w’ubuvuzi bwa kanseri yibara, cyerekanaga ko ahantu hahindurwa mutation itera K-ras gukora cyane cyane muri kode ya 12 na 13 za exon 2, maze isaba ko abarwayi bose barwaye kanseri yu mura yateye imbere barashobora gupimwa ihinduka rya K-ras mbere yo kuvurwa.Kubwibyo, kumenya vuba na neza K-ras ihindagurika rya gene bifite akamaro kanini mubuyobozi bwo kuvura imiti.Iki gikoresho gikoresha ADN nk'icyitegererezo cyo gutahura kugira ngo gitange isuzuma ryujuje ubuziranenge bw'imiterere ya mutation, ishobora gufasha abaganga gusuzuma kanseri y'urura runini, kanseri y'ibihaha ndetse n'abandi barwayi b'ibibyimba bungukirwa n'ibiyobyabwenge.Ibisubizo by'ibizamini by'ibikoresho ni ibyavuzwe gusa kandi ntibigomba gukoreshwa nk'ishingiro ryonyine ryo kuvura abarwayi ku giti cyabo.Abaganga b’amavuriro bagomba gufata imyanzuro yuzuye kubisubizo by’ibizamini hashingiwe ku bintu nk’umurwayi umeze, ibimenyetso by’ibiyobyabwenge, igisubizo cy’ubuvuzi n’ibindi bipimo bya laboratoire.

Ibipimo bya tekiniki

Ububiko Amazi: ≤-18 ℃ Mu mwijima;Lyophilized: ≤30 ℃ Mu mwijima
Ubuzima bwa Shelf Amazi: amezi 9;Lyophilized: amezi 12
Ubwoko bw'icyitegererezo paraffin-yashyizwemo na pathologiya tissue cyangwa igice kirimo selile yibibyimba
CV ≤5.0%
LoD K-ras Igisubizo Buffer A na K-ras Igikorwa Buffer B irashobora kumenya neza igipimo cya 1% ihinduka munsi ya 3ng / μL ubwoko bwinyamanswa
Ibikoresho bikoreshwa Ikoreshwa rya Biosystems 7500 Sisitemu-Igihe nyacyo PCR

Ikoreshwa rya Biosystems 7300 Sisitemu-Igihe nyacyo PCR

QuantStudio®5 Sisitemu-Igihe nyacyo PCR

UmucyoCycler® 480 Sisitemu nyayo-PCR

BioRad CFX96 Sisitemu-Igihe Cyuzuye PCR

Urujya n'uruza rw'akazi

Birasabwa gukoresha QIAGEN's QIAamp ADN FFPE Tissue Kit (56404) hamwe na Paraffin yashyizwemo Tissue ADN Rapid Extraction Kit (DP330) yakozwe na Tiangen Biotech (Beijing) Co., Ltd.


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