Umuntu Leukocyte Antigen B27 Acide Nucleic

Ibisobanuro bigufi:

Iki gikoresho gikoreshwa mugushakisha ADN yujuje ubuziranenge ubwoko bwa leukocyte antigen yumuntu HLA-B * 2702, HLA-B * 2704 na HLA-B * 2705.


Ibicuruzwa birambuye

Ibicuruzwa

Izina RY'IGICURUZWA

HWTS-GE011A-Umuntu Leukocyte Antigen B27 Igikoresho cyo Kumenya Acide Nucleic (Fluorescence PCR)

Epidemiologiya

Ankylose spondylitis (AS) n'indwara idakira itera indwara yibasira cyane urutirigongo kandi ishobora kuba irimo ingingo ya sacroiliac hamwe nibice bikikije kuburyo butandukanye.Byagaragaye ko AS igaragaza umuryango ugaragara kandi ko ifitanye isano rya bugufi na antigen ya leukocyte ya muntu HLA-B27.Mu bantu, ubwoko burenga 70 bwubwoko bwa HLA-B27 bwaravumbuwe kandi buramenyekana, kandi muribo, HLA-B * 2702, HLA-B * 2704 na HLA-B * 2705 nubwoko bukunze kugaragara bujyanye niyi ndwara.Mu Bushinwa, Singapuru, Ubuyapani n'akarere ka Tayiwani mu Bushinwa, ubwoko bukunze kugaragara bwa HLA-B27 ni HLA-B * 2704, bugera kuri 54%, bukurikirwa na HLA-B * 2705, bingana na 41%.Iki gikoresho gishobora kumenya ADN muburyo butandukanye HLA-B * 2702, HLA-B * 2704 na HLA-B * 2705, ariko ntibitandukanya.

Umuyoboro

FAM HLA-B27
VIC / HEX Igenzura ryimbere

Ibipimo bya tekiniki

Ububiko Amazi: ≤-18 ℃ Mu mwijima
Ubuzima bwa Shelf Amazi: amezi 18
Ubwoko bw'icyitegererezo amaraso yose
Ct ≤40
CV ≤5.0%
LoD 1ng / μL

Umwihariko

 

Ibisubizo by'ibizamini byabonetse muri iki gikoresho ntibizaterwa na hemoglobine (<800g / L), bilirubin (<700μmol / L), na lipide y'amaraso / triglyceride (<7mmol / L) mu maraso.
Ibikoresho bikoreshwa Ikoreshwa rya Biosystems 7500 Sisitemu-Igihe nyacyo PCR

Ikoreshwa rya Biosystems Intambwe imwe Yukuri-Igihe PCR Sisitemu

LightCycler®480 Sisitemu nyayo-PCR

Agilent-Stratagene Mx3000P Q-PCR Sisitemu


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