Umuntu BRAF Gene V600E Guhinduka

Ibisobanuro bigufi:

Iki gikoresho cyo kwipimisha gikoreshwa kugirango hamenyekane neza ihinduka rya BRAF gene V600E ihindagurika ryimiterere ya paraffin yashizwemo ingero za melanoma yumuntu, kanseri yibara, kanseri ya tiroyide na kanseri yibihaha muri vitro.


Ibicuruzwa birambuye

Ibicuruzwa

Izina RY'IGICURUZWA

HWTS-TM007-BRAF Yumuntu Gene V600E Igikoresho cyo Guhindura Mutation (Fluorescence PCR)

Icyemezo

CE

Epidemiologiya

Habonetse ubwoko burenga 30 bwimiterere ya BRAF, muribo hafi 90% biherereye muri exon 15, aho ihinduka rya V600E rifatwa nkimihindagurikire ikunze kugaragara, ni ukuvuga thymine (T) kumwanya wa 1799 muri exon 15 ihinduka kuri adenine (A), bivamo gusimbuza valine (V) kumwanya wa 600 na acide glutamic (E) mubicuruzwa bya poroteyine.Guhinduka kwa BRAF bikunze kuboneka mu bibyimba bibi nka melanoma, kanseri yibara, kanseri ya tiroyide, na kanseri y'ibihaha.Gusobanukirwa ihinduka ryimiterere ya gene ya BRAF byabaye nkenerwa gusuzuma EGFR-TKIs na BRAF gene yibasiwe n’imiti igamije kuvura imiti ivura abarwayi bashobora kugirirwa akamaro.

Umuyoboro

FAM V600E ihinduka, kugenzura imbere

Ibipimo bya tekiniki

Ububiko

≤-18 ℃

Ubuzima bwa Shelf

Amezi 9

Ubwoko bw'icyitegererezo

paraffin-yashyizwemo na patologue tissue sample

CV

< 5.0%

Ct

≤38

LoD

Koresha ibikoresho kugirango umenye igenzura ryiza rya LoD.a) munsi ya 3ng / μL ubwoko bwimiterere yinyamanswa, igipimo cya 1% cya mutation kirashobora kugaragara muri buffer reaction;b) munsi ya 1% igipimo cya mutation, ihinduka rya 1 × 103Amakopi / mL muburyo bwimiterere yinyamanswa ya 1 × 105Amakopi / mL arashobora kugaragara neza muri buffer reaction;c) IC Reaction Buffer irashobora kumenya igipimo ntarengwa cyo kugenzura ubuziranenge SW3 yo kugenzura imbere mu kigo.

Ibikoresho bikoreshwa:

Ikoreshwa rya Biosystems 7500 Sisitemu-Igihe nyacyo PCRIkoreshwa rya Biosystems 7300 Igihe-nyacyo PCR

Sisitemu, QuantStudio® 5 Sisitemu-Igihe Cyuzuye PCR

LightCycler®480 Sisitemu nyayo-PCR

BioRad CFX96 Sisitemu-Igihe Cyuzuye PCR

Urujya n'uruza rw'akazi

Gusabwa gukuramo reagent: QIAGEN ya QIAamp ADN FFPE Tissue Kit (56404), Paraffin yashyizwemo Tissue ADN Rapid Extraction Kit (DP330) yakozwe na Tiangen Biotech (Beijing) Co., Ltd.


  • Mbere:
  • Ibikurikira:

  • Andika ubutumwa bwawe hano hanyuma utwohereze