Umuntu BRAF Gene V600E Guhinduka
Izina RY'IGICURUZWA
HWTS-TM007-BRAF Yumuntu Gene V600E Igikoresho cyo Guhindura Mutation (Fluorescence PCR)
Icyemezo
CE
Epidemiologiya
Habonetse ubwoko burenga 30 bwimiterere ya BRAF, muribo hafi 90% biherereye muri exon 15, aho ihinduka rya V600E rifatwa nkimihindagurikire ikunze kugaragara, ni ukuvuga thymine (T) kumwanya wa 1799 muri exon 15 ihinduka kuri adenine (A), bivamo gusimbuza valine (V) kumwanya wa 600 na acide glutamic (E) mubicuruzwa bya poroteyine.Guhinduka kwa BRAF bikunze kuboneka mu bibyimba bibi nka melanoma, kanseri yibara, kanseri ya tiroyide, na kanseri y'ibihaha.Gusobanukirwa ihinduka ryimiterere ya gene ya BRAF byabaye nkenerwa gusuzuma EGFR-TKIs na BRAF gene yibasiwe n’imiti igamije kuvura imiti ivura abarwayi bashobora kugirirwa akamaro.
Umuyoboro
| FAM | V600E ihinduka, kugenzura imbere |
Ibipimo bya tekiniki
| Ububiko | ≤-18 ℃ |
| Ubuzima bwa Shelf | Amezi 9 |
| Ubwoko bw'icyitegererezo | paraffin-yashyizwemo na patologue tissue sample |
| CV | ＜ 5.0% |
| Ct | ≤38 |
| LoD | Koresha ibikoresho kugirango umenye igenzura ryiza rya LoD.a) munsi ya 3ng / μL ubwoko bwimiterere yinyamanswa, igipimo cya 1% cya mutation kirashobora kugaragara muri buffer reaction;b) munsi ya 1% igipimo cya mutation, ihinduka rya 1 × 103Amakopi / mL muburyo bwimiterere yinyamanswa ya 1 × 105Amakopi / mL arashobora kugaragara neza muri buffer reaction;c) IC Reaction Buffer irashobora kumenya igipimo ntarengwa cyo kugenzura ubuziranenge SW3 yo kugenzura imbere mu kigo. |
| Ibikoresho bikoreshwa: | Ikoreshwa rya Biosystems 7500 Sisitemu-Igihe nyacyo PCRIkoreshwa rya Biosystems 7300 Igihe-nyacyo PCR Sisitemu, QuantStudio® 5 Sisitemu-Igihe Cyuzuye PCR LightCycler®480 Sisitemu nyayo-PCR BioRad CFX96 Sisitemu-Igihe Cyuzuye PCR |
Urujya n'uruza rw'akazi
Gusabwa gukuramo reagent: QIAGEN ya QIAamp ADN FFPE Tissue Kit (56404), Paraffin yashyizwemo Tissue ADN Rapid Extraction Kit (DP330) yakozwe na Tiangen Biotech (Beijing) Co., Ltd.
