Virusi ya Coxsackie Ubwoko A16 Acide Nucleic

Ibisobanuro bigufi:

Iki gikoresho gikoreshwa muri vitro yujuje ubuziranenge bwa virusi ya Coxsackie yo mu bwoko bwa A16 nucleic aside mu muhogo wabantu.


Ibicuruzwa birambuye

Ibicuruzwa

Izina RY'IGICURUZWA

HWTS-EV025-Virusi ya Coxsackie Ubwoko A16 Igikoresho cyo Kumenya Acide Nucleic (Enzymatic Probe Isothermal Amplification)

Icyemezo

CE

Epidemiologiya

Iki gikoresho gikoresha Enzymatic Probe Isothermal Amplification (EPIA), ikanashushanya primers yihariye hamwe na RNA ishingiye kuri probe (rProbe) mukarere kabungabunzwe cyane ka Cox A16, ikongeramo enzyme ya Bst na enzyme ya RNaseH icyarimwe, aho ibumoso na ibumoso Impera yiburyo ya RNA ishingiro rya rProbe yanditseho amatsinda ya fluorescent hamwe na quencher.Koresha ibikorwa bya ADN polymerase hamwe nigikorwa cyo kwimura imirongo ya Bst enzyme kugirango wongere intego igeragezwa mubushyuhe burigihe, enzyme ya RNaseH irashobora guhanagura ibirindiro bya RNA kumurongo wa Hybrid, kugirango itsinda rya fluorescent hamwe no kuzimya rProbe. gutandukana bityo fluorescing.Mubyongeyeho, igice cyibumoso cyibisigisigi bya rProbe RNA gishobora gukoreshwa nka primer kugirango irusheho kwaguka kugirango ikore ibicuruzwa, bikomeza kwegeranya ibicuruzwa.Ikimenyetso cya fluorescent gikusanyirizwa hamwe hamwe no gukora ibicuruzwa, bityo bikamenya kumenya aside nucleic igenewe.

Umuyoboro

FAM Ubwoko bwa virusi ya Coxsackie A16
ROX impaka imbere

Ibipimo bya tekiniki

Ububiko

≤-18 ℃

Ubuzima bwa Shelf

Amezi 12

Ubwoko bw'icyitegererezo

Gukusanya umuhogo mushya

CV

≤10.0%

Ct

≤38

LoD

2000Kopi / mL

Ibikoresho bikoreshwa

Ikoreshwa rya Biosystems 7500 Sisitemu-Igihe nyacyo PCRSLAN-96P Sisitemu nyayo-PCR

LightCycler®480 Sisitemu nyayo-PCR

Igihe nyacyo Fluorescence Yama Sisitemu Yerekana Ubushyuhe Bworoshye Amp HWTS1600

Urujya n'uruza rw'akazi

Ihitamo 1

Gusabwa gukuramo reagent: Macro & Micro-Ikizamini cya virusi ADN / RNA Kit (HWTS-3001, HWTS-3004-32, HWTS-3004-48) na Macro & Micro-Test Automatic Nucleic Acide (HWTS-3006)

Icya 2

Basabwe gukuramo reagent: Macro & Micro-Ikigereranyo Cyitegererezo cyo Gusohora Reagent (HWTS-3005-8).


  • Mbere:
  • Ibikurikira:

  • Andika ubutumwa bwawe hano hanyuma utwohereze