Virusi ya Coxsackie Ubwoko A16 Acide Nucleic

Iki gikoresho gikoresha Enzymatic Probe Isothermal Amplification (EPIA), ikanashushanya primers yihariye hamwe na RNA ishingiye kuri probe (rProbe) mukarere kabungabunzwe cyane ka Cox A16, ikongeramo enzyme ya Bst na enzyme ya RNaseH icyarimwe, aho ibumoso na ibumoso Impera yiburyo ya RNA ishingiro rya rProbe yanditseho amatsinda ya fluorescent hamwe na quencher.Koresha ibikorwa bya ADN polymerase hamwe nigikorwa cyo kwimura imirongo ya Bst enzyme kugirango wongere intego igeragezwa mubushyuhe burigihe, enzyme ya RNaseH irashobora guhanagura ibirindiro bya RNA kumurongo wa Hybrid, kugirango itsinda rya fluorescent hamwe no kuzimya rProbe. gutandukana bityo fluorescing.Mubyongeyeho, igice cyibumoso cyibisigisigi bya rProbe RNA gishobora gukoreshwa nka primer kugirango irusheho kwaguka kugirango ikore ibicuruzwa, bikomeza kwegeranya ibicuruzwa.Ikimenyetso cya fluorescent gikusanyirizwa hamwe hamwe no gukora ibicuruzwa, bityo bikamenya kumenya aside nucleic igenewe.