Mycobacterium Igituntu Isoniazid Kurwanya Mutation

Ibisobanuro bigufi:

Iki gikoresho kirakwiriye kugirango hamenyekane neza ihinduka ry’ibanze ry’imihindagurikire y’imyororokere y’abantu yakusanyirijwe mu barwayi beza ba Tubercle bacillus itera indwara ya mycobacterium igituntu isoniazid irwanya: Akarere ka InhA gashinzwe -15C> T, -8T> A, -8T> C;AhpC iteza imbere akarere -12C> T, -6G> A;ihinduka ryimibonano mpuzabitsina ya KatG 315 codon 315G> A, 315G> C.

Ohereza imeri kuri twe

Ibicuruzwa birambuye

Ibicuruzwa

Izina RY'IGICURUZWA

HWTS-RT137 Mycobacterium Igituntu Isoniazid Kurwanya Kurwanya Mutation Igikoresho (Gushonga umurongo)

Epidemiologiya

Mycobacterium igituntu, mugihe gito nka Tubercle bacillus (TB), ni bagiteri itera indwara yigituntu.Kugeza ubu, imiti ikoreshwa cyane ku murongo wa mbere wo kurwanya igituntu harimo isoniazid, rifampicin na hexambutol, n'ibindi. Imiti yo ku murongo wa kabiri irwanya igituntu irimo fluoroquinolone, amikacin na kanamycin, n'ibindi. . Icyakora, kubera gukoresha nabi imiti irwanya igituntu hamwe nibiranga imiterere y'urukuta rw'akagari ka mycobacterium igituntu, mycobacterium igituntu itera imiti igabanya imiti igabanya ubukana, izana ibibazo bikomeye mu gukumira no kuvura igituntu.

Umuyoboro

FAM	MP aside nucleic
ROX	Igenzura ryimbere

Ibipimo bya tekiniki

Ububiko	≤-18 ℃
Ubuzima bwa Shelf	Amezi 12
Ubwoko bw'icyitegererezo	ururenda
CV	≤5.0%
LoD	Umupaka wo gutahura ubwoko bwa isoniazide yo mu bwoko bwa gasozi ni bacteri 2x103 / mL, naho igipimo cyo kumenya bagiteri za mutant ni bacteri 2x103 / mL.
Umwihariko	a.Nta reaction yambukiranya genome yabantu, izindi mycobacteria nontuberculous mycobacteria na pneumonia patoggen zagaragajwe niki gitabo. b.Ahantu hagaragaye ihinduka ry’imiterere y’indi miti irwanya ibiyobyabwenge mu bwoko bw’igituntu cyitwa Mycobacterium igituntu, nk’imyigaragambyo igena akarere ka gene ya rifampicin rpoB, byagaragaye, kandi ibisubizo by’ibizamini byagaragaje ko bitarwanya isoniazide, byerekana ko nta reaction y’umusaraba.
Ibikoresho bikoreshwa	SLAN-96P Sisitemu nyayo-PCR BioRad CFX96 Sisitemu-Igihe Cyukuri PCR UmucyoCycler480® Sisitemu nyayo-PCR

Urujya n'uruza rw'akazi

Niba ukoresha Macro & Micro-Ikizamini Rusange ADN / RNA Kit (HWTS-3019) (ishobora gukoreshwa na Macro & Micro-Test Automatic Nucleic Acide Extractor (HWTS-3006C, HWTS-3006B)) na Jiangsu Macro & Micro-Ikizamini Med-Tech Co, Ltd yo gukuramo, ongeramo 200μL yo kugenzura nabi no gutunganya spumum sample kugirango igerageze mukurikirane, hanyuma wongereho 10μL yo kugenzura imbere imbere muburyo butandukanye bwo kugenzura nabi, gutunganya intanga ngabo kugirango bipimwe, kandi intambwe ikurikiraho igomba gukorwa cyane ukurikije amabwiriza yo gukuramo.Ingano yicyitegererezo yakuweho ni 200μL, kandi icyifuzo cyo gukuraho ni 100μL.