Umuntu Papillomavirus (Ubwoko 28) Genotyping
Izina RY'IGICURUZWA
HWTS-CC013-Papillomavirus Yabantu (Ubwoko 28) Igikoresho cyo Gutahura Genotyping (Fluorescence PCR)
Epidemiologiya
Kanseri y'inkondo y'umura ni kimwe mu bibyimba bikunze kugaragara mu myanya myororokere y'abagore.Ubushakashatsi bwakozwe mbere bwerekanye ko kwandura no kwandura indwara nyinshi za papillomavirus ari imwe mu mpamvu zitera kanseri y'inkondo y'umura.Kugeza ubu, haracyari ikibazo cyo kuvura uburyo bwiza bwo kuvura HPV, bityo kumenya hakiri kare no kwirinda hakiri kare HPV y'inkondo y'umura ni urufunguzo rwo guhagarika kanseri.Gushiraho uburyo bworoshye, bwihariye kandi bwihuse bwo gusuzuma indwara ya etiologiya bifite akamaro kanini mugupima kanseri y'inkondo y'umura.
Umuyoboro
| Buffer reaction | FAM | VIC / HEX | ROX | CY5 |
| HPV Ubwoko bwa Genotyping reaction Buffer 1 | 16 | 18 | / | Kugenzura imbere |
| HPV Genotyping Reaction Buffer 2 | 56 | / | 31 | Kugenzura imbere |
| HPV Genotyping Reaction Buffer 3 | 58 | 33 | 66 | 35 |
| HPV Genotyping Reaction Buffer 4 | 53 | 51 | 52 | 45 |
| HPV Genotyping Reaction Buffer 5 | 73 | 59 | 39 | 68 |
| HPV Genotyping Reaction Buffer 6 | 6 | 11 | 83 | 54 |
| HPV Genotyping Reaction Buffer 7 | 26 | 44 | 61 | 81 |
| HPV Genotyping Reaction Buffer 8 | 40 | 43 | 42 | 82 |
Ibipimo bya tekiniki
| Ububiko | Amazi: ≤-18 ℃ |
| Ubuzima bwa Shelf | Amezi 12 |
| Ubwoko bw'icyitegererezo | inkondo y'umura |
| Ct | ≤28 |
| CV | ≤5.0% |
| LoD | 300Kopi / mL |
| Ibikoresho bikoreshwa | SLAN®-96P Sisitemu nyayo-PCR Ikoreshwa rya Biosystems 7500 Sisitemu-Igihe nyacyo PCR QuantStudio®5 Sisitemu-Igihe nyacyo PCR LineGene 9600 Yongeyeho Sisitemu-Igihe Cyuzuye PCR MA-6000-Igihe-Cyuzuye Cyumubare Wubushyuhe |
Urujya n'uruza rw'akazi
Ihitamo 1.
Basabwe gukuramo reagent: Macro & Micro-Ikigereranyo Cyitegererezo cyo Gusohora Reagent (HWTS-3005-8)
Icya 2.
Gusabwa gukuramo reagent: Macro & Micro-Ikizamini cya virusi ADN / RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) na Macro & Micro-Test Automatic Nucleic Acide (HWTS-3006C, HWTS- 3006B)
