Papillomavirus Yumuntu (Ubwoko 28) Genotyping Detection Kit (Fluorescence PCR)

Igikoresho gikoresha aside nucleic aside amplification (PCR) uburyo bwo kumenya fluorescence.Byibanze cyane primers na probe byateguwe hashingiwe kuri L1 gene ikurikirana ya HPV.Iperereza ryihariye ryanditseho fluorophore ya FAM (HPV6, 16, 26, 40, 53, 58, 73), fluorophore ya VIC / HEX (HPV11, 18, 33, 43, 51, 59, 81), fluorofore CY5 (HPV35, 44 , 45, 54, 56, 68, 82) na fluorophore ya ROX (HPV31, 39, 42, 52, 61, 66, 83) kuri 5 ', naho itsinda rya 3' kuzimya ni BHQ1 cyangwa BHQ2.Mugihe cyo kongera PCR, primers na probe bihuza intego zabo zikurikirana.Iyo Taq enzyme ihuye nubushakashatsi bujyanye nurutonde rwakorewe, ikora umurimo wa 5 'end exonuclease kugirango itandukane umunyamakuru fluorophore na fluorophore ya quencher, kugirango sisitemu yo gukurikirana fluorescence ibone ibimenyetso bya fluorescente, ni ukuvuga igihe cyose ADN umurongo wongerewe imbaraga, hashyizweho molekile ya fluorescente, ikamenya guhuza byimazeyo gukusanya ibimenyetso bya fluorescent no gushiraho ibicuruzwa bya PCR, kugirango bigerweho kandi byujuje ubuziranenge bwa genoside ya acide nucleic yubwoko 28 bwa papillomavirus yumuntu wintangangore zifata ingirabuzimafatizo. .