15 Ubwoko bwibyago byinshi Umuntu Papillomavirus E6 / E7 Gene mRNA
Izina RY'IGICURUZWA
HWTS-CC005A-15 Ubwoko bwibyago byinshi byabantu Papillomavirus E6 / E7 Gene mRNA Igikoresho cyo kumenya (Fluorescence PCR)
Epidemiologiya
Kanseri y'inkondo y'umura ni bumwe mu bwoko bwa kanseri y'abagore ku isi hose, kandi kuba bibaho bifitanye isano rya bugufi na papillomavirusi y'abantu (HPV), ariko umubare muto w'abanduye HPV ushobora kwandura kanseri.HPV ifite ibyago byinshi yanduza kanseri y'inkondo y'umura kandi ikabyara oncoproteine ebyiri, E6 na E7.Iyi poroteyine irashobora kugira ingaruka kuri poroteyine zitandukanye za selile (nka proteine suppressor proteine pRB na p53), ikongerera ingirabuzimafatizo, ikagira ingaruka kuri synthesis ya ADN hamwe na genome itajegajega, kandi ikabangamira imiti irwanya virusi na antitumor.
Umuyoboro
| Umuyoboro | Ibigize | Genotype yageragejwe |
| FAM | HPV Igisubizo Buffer 1 | HPV16、31、33、35、51、52、58 |
| VIC / HEX | Umuntu β-actin gene | |
| FAM | HPV Imyitwarire ya Buffer 2 | HPV 18、39、45、53、56、59、66、68 |
| VIC / HEX | Umuntu INS |
Ibipimo bya tekiniki
| Ububiko | Amazi: ≤-18 ℃ |
| Ubuzima bwa Shelf | Amezi 9 |
| Ubwoko bw'icyitegererezo | inkondo y'umura |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | Amakopi 500 / mL |
| Ibikoresho bikoreshwa | Ikoreshwa rya Biosystems 7500 Sisitemu-Igihe nyacyo PCR Ikoreshwa rya Biosystems 7500 Byihuse-Igihe-Sisitemu ya PCR QuantStudio®5 Sisitemu-Igihe nyacyo PCR SLAN-96P Sisitemu nyayo-PCR LightCycler®480 Sisitemu nyayo-PCR LineGene 9600 Yongeyeho Sisitemu-Igihe Cyuzuye PCR MA-6000-Igihe-Cyuzuye Cyumubare Wubushyuhe BioRad CFX96 Sisitemu-Igihe Cyuzuye PCR BioRad CFX Opus 96 Sisitemu Yigihe-PCR |
Urujya n'uruza rw'akazi
Gusabwa gukuramo reagent: Macro & Micro-Test Virus ADN / RNA Kit (HWTS-3020-50-HPV15) na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Gukuramo bigomba gukorwa cyane ukurikije amabwiriza yo gukoresha. .Ingano isabwa yo gukuraho ni 50μL.Niba icyitegererezo kitarimo neza, subiza kumurongo wa 4 kugirango wongere ugarure.Hanyuma hanyuma ugerageze ukurikije amabwiriza yo gukoresha.
Gusabwa gukuramo reagent: RNAprep Inyamanswa Yinyamanswa Yuzuye Igikoresho cyo gukuramo RNA (DP431).Gukuramo bigomba gukorwa hakurikijwe amabwiriza yo gukoresha byimazeyo (Muntambwe ya 5, wikubye kabiri igisubizo cyakazi cya DNaseI, ni ukuvuga, fata 20μL ya RNase-DNaseI (1500U) igisubizo cyibigega mumashanyarazi mashya ya RNase-yubusa, ongeramo 60μL ya buffer ya RDD, hanyuma uvange witonze).Ingano isabwa yo gukuraho ni 60μL.Niba icyitegererezo kitarimo neza, subiza kuri intambwe ya 5 kugirango wongere ugarure.Hanyuma hanyuma ugerageze ukurikije amabwiriza yo gukoresha.
